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For breeding, 2 male and 1 female adult zebrafish were randomly selected from home aquariums and placed in breeding tanks (approximately at 5 pm) with a grid specifically designed to prevent egg predation by separating the adults from the embryos

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Carotenoid estimation In the acetone extract, control callus showed the lowest content (1.65±0.03 µg g -1 FW), which increased significantly following treatments with ZnONPs, reaching to 7.73±0.24 µg g -1 FW at 2 mg L -1